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BPS Bioscience recombinant dnmt protein
a FPCK-1-1 cells were treated with 100 nM DBIC, DBIC-neg1, or 126 μM NOC18 every 3 days for a month before transfer to 3D culture. Spheroidal aggregate formation of FPCK-1-1 (or FPCKpP1-4 control) cells was assessed 3 d after plating. Scale bar, 50 μm. The data represent one of four separate experiments. b DBIC inhibited NO-induced sphere formation assessed 7 days post plating. Values are mean ± s.e.m. ( n = 4; *** P < 0.001 by two-way ANOVA with Bonferroni’s post hoc test). Treatments: None (black); vehicle (blue); DBIC (purple); DBIC-neg1 (green); NO (white); NO + DBIC (red); NO + DBIC-neg1 (orange). Colors correspond to code in representative micrographs in ( a ). c DBIC inhibits inflammation-related carcinogenesis in vivo. DBIC, DBIC-neg1 (25 mg/kg/day), or 1400 W (6 mg/kg/day) administered by i.p. injection from pre-implantation day 2 through day 35 post cell implantation. Regressive QR-32 cells (1 × 10 5 ) were implanted into mice on day 0 in a subcutaneously pre-inserted foreign body (gelatin sponge, 10 × 5 × 3 mm). ** P < 0.01, *** P < 0.001 vs. vehicle by χ 2 -test. d Time course of DBIC concentration in plasma after single i.p. administration (25 mg/kg) measured by LC-MS/MS. Values are mean ± s.e.m. ( n = 3). e Concentration of DBIC i n tumor tissue after 25 d of daily administration. Samples were analyzed 1 h after the final dose. For box plots, center line represents median; box limits are 25th and 75th percentiles. Whiskers represent minimum-maximum values ( n = 10). f SNO-DNMT3B in tumor tissue. Values are mean ± s.e.m. ( n = 3; ** P < 0.01 by two-tailed Student’s t -test). g Relative ratio of SNO-DNMT3B in human and mouse tumors. Biotin-switch and immunoblot assays were quantified by densitometry to calculate relative ratio of SNO-DNMT3B to total DNMT3B. Values are mean ± s.e.m. ( n = 6–7; * P < 0.05, ** P < 0.01 vs Normal by o n e-way ANOVA with uncorrected Fisher’s LSD post hoc test). h Schematic of <t>SNO-DNMT</t> mechanism of action on expression of specific genes associated with neoplasia via decreased methylation <t>of</t> <t>CpG</t> sites. Source data are provided as a Source data file.
Recombinant Dnmt Protein, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a FPCK-1-1 cells were treated with 100 nM DBIC, DBIC-neg1, or 126 μM NOC18 every 3 days for a month before transfer to 3D culture. Spheroidal aggregate formation of FPCK-1-1 (or FPCKpP1-4 control) cells was assessed 3 d after plating. Scale bar, 50 μm. The data represent one of four separate experiments. b DBIC inhibited NO-induced sphere formation assessed 7 days post plating. Values are mean ± s.e.m. ( n = 4; *** P < 0.001 by two-way ANOVA with Bonferroni’s post hoc test). Treatments: None (black); vehicle (blue); DBIC (purple); DBIC-neg1 (green); NO (white); NO + DBIC (red); NO + DBIC-neg1 (orange). Colors correspond to code in representative micrographs in ( a ). c DBIC inhibits inflammation-related carcinogenesis in vivo. DBIC, DBIC-neg1 (25 mg/kg/day), or 1400 W (6 mg/kg/day) administered by i.p. injection from pre-implantation day 2 through day 35 post cell implantation. Regressive QR-32 cells (1 × 10 5 ) were implanted into mice on day 0 in a subcutaneously pre-inserted foreign body (gelatin sponge, 10 × 5 × 3 mm). ** P < 0.01, *** P < 0.001 vs. vehicle by χ 2 -test. d Time course of DBIC concentration in plasma after single i.p. administration (25 mg/kg) measured by LC-MS/MS. Values are mean ± s.e.m. ( n = 3). e Concentration of DBIC i n tumor tissue after 25 d of daily administration. Samples were analyzed 1 h after the final dose. For box plots, center line represents median; box limits are 25th and 75th percentiles. Whiskers represent minimum-maximum values ( n = 10). f SNO-DNMT3B in tumor tissue. Values are mean ± s.e.m. ( n = 3; ** P < 0.01 by two-tailed Student’s t -test). g Relative ratio of SNO-DNMT3B in human and mouse tumors. Biotin-switch and immunoblot assays were quantified by densitometry to calculate relative ratio of SNO-DNMT3B to total DNMT3B. Values are mean ± s.e.m. ( n = 6–7; * P < 0.05, ** P < 0.01 vs Normal by o n e-way ANOVA with uncorrected Fisher’s LSD post hoc test). h Schematic of <t>SNO-DNMT</t> mechanism of action on expression of specific genes associated with neoplasia via decreased methylation <t>of</t> <t>CpG</t> sites. Source data are provided as a Source data file.
Quick Pcr Cloning Kit, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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G Biosciences z-competent cell kit
a FPCK-1-1 cells were treated with 100 nM DBIC, DBIC-neg1, or 126 μM NOC18 every 3 days for a month before transfer to 3D culture. Spheroidal aggregate formation of FPCK-1-1 (or FPCKpP1-4 control) cells was assessed 3 d after plating. Scale bar, 50 μm. The data represent one of four separate experiments. b DBIC inhibited NO-induced sphere formation assessed 7 days post plating. Values are mean ± s.e.m. ( n = 4; *** P < 0.001 by two-way ANOVA with Bonferroni’s post hoc test). Treatments: None (black); vehicle (blue); DBIC (purple); DBIC-neg1 (green); NO (white); NO + DBIC (red); NO + DBIC-neg1 (orange). Colors correspond to code in representative micrographs in ( a ). c DBIC inhibits inflammation-related carcinogenesis in vivo. DBIC, DBIC-neg1 (25 mg/kg/day), or 1400 W (6 mg/kg/day) administered by i.p. injection from pre-implantation day 2 through day 35 post cell implantation. Regressive QR-32 cells (1 × 10 5 ) were implanted into mice on day 0 in a subcutaneously pre-inserted foreign body (gelatin sponge, 10 × 5 × 3 mm). ** P < 0.01, *** P < 0.001 vs. vehicle by χ 2 -test. d Time course of DBIC concentration in plasma after single i.p. administration (25 mg/kg) measured by LC-MS/MS. Values are mean ± s.e.m. ( n = 3). e Concentration of DBIC i n tumor tissue after 25 d of daily administration. Samples were analyzed 1 h after the final dose. For box plots, center line represents median; box limits are 25th and 75th percentiles. Whiskers represent minimum-maximum values ( n = 10). f SNO-DNMT3B in tumor tissue. Values are mean ± s.e.m. ( n = 3; ** P < 0.01 by two-tailed Student’s t -test). g Relative ratio of SNO-DNMT3B in human and mouse tumors. Biotin-switch and immunoblot assays were quantified by densitometry to calculate relative ratio of SNO-DNMT3B to total DNMT3B. Values are mean ± s.e.m. ( n = 6–7; * P < 0.05, ** P < 0.01 vs Normal by o n e-way ANOVA with uncorrected Fisher’s LSD post hoc test). h Schematic of <t>SNO-DNMT</t> mechanism of action on expression of specific genes associated with neoplasia via decreased methylation <t>of</t> <t>CpG</t> sites. Source data are provided as a Source data file.
Z Competent Cell Kit, supplied by G Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a FPCK-1-1 cells were treated with 100 nM DBIC, DBIC-neg1, or 126 μM NOC18 every 3 days for a month before transfer to 3D culture. Spheroidal aggregate formation of FPCK-1-1 (or FPCKpP1-4 control) cells was assessed 3 d after plating. Scale bar, 50 μm. The data represent one of four separate experiments. b DBIC inhibited NO-induced sphere formation assessed 7 days post plating. Values are mean ± s.e.m. ( n = 4; *** P < 0.001 by two-way ANOVA with Bonferroni’s post hoc test). Treatments: None (black); vehicle (blue); DBIC (purple); DBIC-neg1 (green); NO (white); NO + DBIC (red); NO + DBIC-neg1 (orange). Colors correspond to code in representative micrographs in ( a ). c DBIC inhibits inflammation-related carcinogenesis in vivo. DBIC, DBIC-neg1 (25 mg/kg/day), or 1400 W (6 mg/kg/day) administered by i.p. injection from pre-implantation day 2 through day 35 post cell implantation. Regressive QR-32 cells (1 × 10 5 ) were implanted into mice on day 0 in a subcutaneously pre-inserted foreign body (gelatin sponge, 10 × 5 × 3 mm). ** P < 0.01, *** P < 0.001 vs. vehicle by χ 2 -test. d Time course of DBIC concentration in plasma after single i.p. administration (25 mg/kg) measured by LC-MS/MS. Values are mean ± s.e.m. ( n = 3). e Concentration of DBIC i n tumor tissue after 25 d of daily administration. Samples were analyzed 1 h after the final dose. For box plots, center line represents median; box limits are 25th and 75th percentiles. Whiskers represent minimum-maximum values ( n = 10). f SNO-DNMT3B in tumor tissue. Values are mean ± s.e.m. ( n = 3; ** P < 0.01 by two-tailed Student’s t -test). g Relative ratio of SNO-DNMT3B in human and mouse tumors. Biotin-switch and immunoblot assays were quantified by densitometry to calculate relative ratio of SNO-DNMT3B to total DNMT3B. Values are mean ± s.e.m. ( n = 6–7; * P < 0.05, ** P < 0.01 vs Normal by o n e-way ANOVA with uncorrected Fisher’s LSD post hoc test). h Schematic of SNO-DNMT mechanism of action on expression of specific genes associated with neoplasia via decreased methylation of CpG sites. Source data are provided as a Source data file.

Journal: Nature Communications

Article Title: Pivotal role for S -nitrosylation of DNA methyltransferase 3B in epigenetic regulation of tumorigenesis

doi: 10.1038/s41467-023-36232-6

Figure Lengend Snippet: a FPCK-1-1 cells were treated with 100 nM DBIC, DBIC-neg1, or 126 μM NOC18 every 3 days for a month before transfer to 3D culture. Spheroidal aggregate formation of FPCK-1-1 (or FPCKpP1-4 control) cells was assessed 3 d after plating. Scale bar, 50 μm. The data represent one of four separate experiments. b DBIC inhibited NO-induced sphere formation assessed 7 days post plating. Values are mean ± s.e.m. ( n = 4; *** P < 0.001 by two-way ANOVA with Bonferroni’s post hoc test). Treatments: None (black); vehicle (blue); DBIC (purple); DBIC-neg1 (green); NO (white); NO + DBIC (red); NO + DBIC-neg1 (orange). Colors correspond to code in representative micrographs in ( a ). c DBIC inhibits inflammation-related carcinogenesis in vivo. DBIC, DBIC-neg1 (25 mg/kg/day), or 1400 W (6 mg/kg/day) administered by i.p. injection from pre-implantation day 2 through day 35 post cell implantation. Regressive QR-32 cells (1 × 10 5 ) were implanted into mice on day 0 in a subcutaneously pre-inserted foreign body (gelatin sponge, 10 × 5 × 3 mm). ** P < 0.01, *** P < 0.001 vs. vehicle by χ 2 -test. d Time course of DBIC concentration in plasma after single i.p. administration (25 mg/kg) measured by LC-MS/MS. Values are mean ± s.e.m. ( n = 3). e Concentration of DBIC i n tumor tissue after 25 d of daily administration. Samples were analyzed 1 h after the final dose. For box plots, center line represents median; box limits are 25th and 75th percentiles. Whiskers represent minimum-maximum values ( n = 10). f SNO-DNMT3B in tumor tissue. Values are mean ± s.e.m. ( n = 3; ** P < 0.01 by two-tailed Student’s t -test). g Relative ratio of SNO-DNMT3B in human and mouse tumors. Biotin-switch and immunoblot assays were quantified by densitometry to calculate relative ratio of SNO-DNMT3B to total DNMT3B. Values are mean ± s.e.m. ( n = 6–7; * P < 0.05, ** P < 0.01 vs Normal by o n e-way ANOVA with uncorrected Fisher’s LSD post hoc test). h Schematic of SNO-DNMT mechanism of action on expression of specific genes associated with neoplasia via decreased methylation of CpG sites. Source data are provided as a Source data file.

Article Snippet: Recombinant DNMT protein (200–400 ng) was used to assess DNA methyltransferase activity against CpG-rich DNA substrates with a DNMT Direct Activity Assay Kit (BPS Biosciences, catalog #52035).

Techniques: In Vivo, Injection, Concentration Assay, Liquid Chromatography with Mass Spectroscopy, Two Tailed Test, Western Blot, Expressing, Methylation